Density Gradient Media (40%, 80%, and 100%)
Description
Density Gradient Media is a ready-to-use, premium solution consisting of silica particles coated with hydrophilic silane, suspended in a balanced salt solution designed to separate highly motile spermatozoa from seminal plasma. Engineered for intrauterine insemination (IUI) and modern andrology workflows, this media exploits cellular density differences to effectively isolate and purify premium-grade, highly functional motile sperm from raw semen samples.Our premium Density Gradient Media provides a highly efficient, biosafe method for processing raw semen ejaculates within Assisted Reproductive Technology (ART) and andrology laboratories. The formulation features high-purity silica particles coated with hydrophilic silane, suspended inside a protective, dual-acting HEPES/Phosphate buffered balanced salt solution. This specialized matrix establishes distinct density boundaries during centrifugation, allowing laboratory technicians to flawlessly separate cells based on their unique physical densities.
During density gradient centrifugation, the medium securely traps immotile sperm, white blood cells, and dead cellular debris within the upper density layers. Meanwhile, highly motile, morphologically healthy spermatozoa successfully migrate through the gradient boundaries to form a clean pellet at the base of the tube. Its primary clinical application is the isolation and purification of premium-grade, highly functional motile sperm from raw semen ejaculate prior to insemination.
To optimize laboratory workflows, the media is supplied as a ready-to-use solution packed in robust, clear bulk square bottles capped with a highly secure white seal closure. Offered in dedicated concentrations of 40% silica gel concentration (ideal for upper solution layering), 80% silica gel concentration (ideal for lower solution layering), and 100% stock solution concentrations, it allows clinics to easily create customized multi-layer gradients tailored to specific patient requirements.
- Silane Coated Hydrophilic Silica Particle Composition.
- HEPES and Phosphate Buffered Balanced Salt Solution Base.
- Ready to use multi-concentration layering stock options.
- Bulk One Hundred Milliliter Transparent Square Bottle Packaging.
- Highly Motile Sperm Isolation and Purification Architecture.
- Strictly Monitored Low Endotoxin Laboratory Safety Standards.
- Advanced Density Separation: Exploits precise cellular density boundaries to effectively isolate top-tier motile spermatozoa from dead cells and seminal plasma.
- Hydrophilic Silane Coating: Formulated with silane-coated silica particles to provide a smooth, frictionless separation matrix that prevents cellular damage.
- Ready-to-Use Bulk Packaging: Supplied pre-formulated in convenient bulk packages of 100 mL, eliminating preparation steps and reducing cleanroom contamination risks.
- Targeted Concentration Options: Available in separate 40%, 80%, and 100% options to facilitate swift, accurate preparation of multi-tier upper and lower gradient layers.
- Dual-Buffered Protection: Suspended inside a highly stable HEPES/Phosphate buffer system to preserve physiological equilibrium outside of an incubator.
- Rigorously Tested Bio-Safety: Certified with extremely low endotoxin levels to ensure maximum protection and safety for human gametes.
- Composition: Silane-coated silica particles in a HEPES/Phosphate buffer.
- Concentrations Available: 40% silica gel concentration, 80% silica gel concentration, and 100% stock solutions.
- Packaging Size: 100 mL bulk packages.
- Bottle Style: Clear square structural bottle with leak-proof white screw cap.
- Osmolality: 290 – 310 mOsm/kg.
- pH Range: 7.3 – 7.5.
- Endotoxin Level: < 0.1 EU/mL (LAL tested).
- Primary Application: Isolation and purification of premium-grade, highly functional motile sperm from raw semen ejaculate prior to IUI insemination.
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